逆转录酶
生物
DNA聚合酶
核苷酸
人类免疫缺陷病毒(HIV)
病毒
病毒学
DNA
核苷酸转移酶
RNA定向DNA聚合酶
遗传学
分子生物学
核糖核酸
基因
作者
Bradley D. Preston,Bernard J. Poiesz,Lawrence A. Loeb
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:1988-11-25
卷期号:242 (4882): 1168-1171
被引量:871
标识
DOI:10.1126/science.2460924
摘要
The human immunodeficiency virus type 1 (HIV-1) shows extensive genetic variation and undergoes rapid evolution. The fidelity of purified HIV-1 reverse transcriptase was measured during DNA polymerization in vitro by means of three different assays. Reverse transcriptase from HIV-1 introduced base-substitution errors in DNA from the bacteriophage phi X174 amber3 at estimated frequencies of 1/2000 to 1/4000. Analyses of misincorporation rates opposite a single template adenine residue showed that HIV-1 reverse transcriptase catalyzed nucleotide mismatches with a specificity of A:C much greater than A:G greater than A:A. The high error rate of HIV-1 reverse transcriptase in vitro translates to approximately five to ten errors per HIV-1 genome per round of replication in vivo. This high error rate suggests that misincorporation by HIV-1 reverse transcriptase is, at least in part, responsible for the hypermutability of the AIDS virus. The specificity of misincorporation may provide a basis for the systematic construction of antiviral nucleosides.
科研通智能强力驱动
Strongly Powered by AbleSci AI