依巴替丁
烟碱激动剂
烟碱乙酰胆碱受体
内科学
胆碱能的
乙酰胆碱受体
重性抑郁障碍
内分泌学
心理学
扣带皮质
乙酰胆碱
双相情感障碍
受体
生物
神经科学
医学
扁桃形结构
中枢神经系统
认知
作者
Andrew S. Gibbons,Kate McPherson,Andrea Gogos,Brian Dean
标识
DOI:10.1016/j.jad.2021.04.007
摘要
We have previously reported reduced expression of the cholinergic autoreceptor CHRM2 in Brodmann's Area (BA) 24 of the anterior cingulate cortex from subjects with major depressive disorder (MDD) and bipolar disorder (BD), consistent with a hypercholinergic state. This led us to investigate whether levels of the high affinity nicotinic acetylcholine receptors are also altered in BA 24. We measured the binding levels of a high-affinity nicotinic receptor-selective radioligand, [3H]epibatidine, in BA 24 from subjects with MDD (n = 20), BD (n = 18) and age- and sex-matched controls (n = 20). We used qPCR to measure mRNA expression of the high affinity nicotinic acetylcholine receptor subunit CHRNB2 in these subjects. [3H]Epibatidine binding density and CHRNB2 mRNA expression were not significantly altered in either MDD or BD compared to control levels. While validating reference genes for our qPCR experiments, we found that the mRNA levels of 3 putative reference genes, TFB1M, PPIA and SNCA, were increased in MDD but not BD compared to controls. Further investigations in other cortical regions showed that these changes were specific to BA24. Cohort size and available patient data were limited due to standard constraints associated with post-mortem studies. Our data suggest that decreased CHRM2 in BA24 in mood disorders is not associated with a corresponding change in high affinity nicotinic acetylcholine receptor expression. Our findings of increased TFB1M, PPIA and SNCA expression in MDD point to a broader derangement of several homeostatic pathways in MDD that are distinct from BD.
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