Research on the Preparation of Oligopeptides with Green Bean Protein Treated by Neutral Protease

[Objective] The green bean protein was hydrolyzed into peptide and amino acid with the neutral protease and the green bean peptide highly hydrolyzed was produced based on the optimal hydrolysis enzymatic condition.[Method] The isolated green bean protein was hydrolyzed with neutral protease to prepare oligopeptide.The orthogonal design with four factors and three levels was applied to optimize condition based on the study single factor.[Results] The result indicated that the optimal reaction condition was the temperature,50 ℃;pH value,6.5; the concentration of substrate,7%;the concentration of enzyme,6 000 U/g and the time of hydrolysis time,240 min.[Conclusion] Under these conditions,average degree of hydrolysis of isolated green bean protein was up to 28.82%.