Structural Identification and Quantitative Analysis of Taxifolin in Larix olgensis Henry var. koreana Nakai

Objective: To identify structurally the taxifolin from Larix olgensis Henry var.koreana Nakai and determinate taxifolin content in the cross section of upper roots of LarixolgensisHenry var.koreana Nakai from Lijiang region of Jilin province and in trees from 11 forest farms.Methods: Chromatographic separation was performed on a 15C18-AR waters column(6 mm × 250 mm,5 μm) with methanol/water(40:60,V/V) as the mobile phase at a flow rate of 1 mL/min.The column temperature was 30 ℃.UV detection was performed at 288 nm.The injection volume was 20 μL.Results: The IR,UV,MS and 13C-NMR data obtained were consistent with those reported in the literature confirming the extract to be taxifolin.A good linear relationship between peak area and taxifolin concentration in the range of 0.05 to 1.0 mg/mL was observed with correlation coefficientr = 0.9998.The average recovery of taxifolin was 99.3% with RSD of 2.6%(n = 9).In upper roots of Larixolgensis Henry var.koreana Nakai,taxifolin was distributed in a gradually decreasing amount from pith to bark and also affected greatly by growth conditions.Conclusion: The analytical method presented in this study proved simple,acurate,specific,repeatable and applicable for quantitative analysis of taxifolin from Larix olgensis Henry var.koreana Nakai.