The Influence of Interleukin-1β and Tumor Necrosis Factor-α on Endothelial Lipase in Rat Aortic Smooth Muscle Cells

Aim To investigate the effects of interleukin-1β(IL-1β) and tumor necrosis factor-α(TNF-α) on endothelial lipase(EL) in rat aortic smooth muscle cell(ASMC).Methods The rat ASMC were obtained from thoracic aortas and cultured by tissue explant method.Passage 3～5 cells were used in experiment,and were randomly divided into 3 groups:GroupⅠ:treated without intervention as the control;GroupⅡ:the rat ASMC were treated in serum-free culture medium containing IL-1β(1 to 30 μg/L) for 24 hours;GroupⅢ:TNF-α(5 to 50 μg/L) for 24 hours.The levels of EL were tested by the method of enzyme linked immunosorbent assay(ELISA) and reverse transcription polymerase chain reaction(RT-PCR).Results Smooth muscle cells were treated with increasing concentrations of IL-1β,ranging from 1 μg/L to 30 μg/L.The relative increases in EL protein and mRNA were greater at the highest concentrations(30 μg/L) of IL-1β.There was a significant difference between the intervention group and the control group(P0.05).There were significant differences among the intervention groups(P0.05).The relative increases in EL protein and mRNA were greater at the highest concentrations(50 μg/L) of TNF-α.There was a significant difference between the intervention group and the control group(P0.05).There were significant differences among the intervention groups(P0.05).Conclusion Smooth muscle cells were treated with increasing concentrations of IL-1β and TNF-α,then secreted protein and EL mRNA were noted.