Different expression of IL-2, IL-10 mRNA in normal pregnancy and recurrent spontaneous abortions.

Objective To examine the expression of the cytokines of Th1(Interleukin 2) and Th2 (Interleukin 10) on the peripheral blood mononuclear cell (PBMC) of normal pregnant women and recurrent spontaneous abortion (RSA) patients. Methods The Interleukin 2 and Interleukin 10 mRNA were determined from PBMCs of 10 normal non pregnant women, 10 normal pregnant women and 10 patients with RSA by semi quantitative reverse transcriptional polymerase chain reaction (RT PCR) method. Results Comparing with the non pregnant women the Interleukin 10 mRNA was higher in normal pregnant women; the Interleukin 10 mRNA was lower, and the Interleukin 2 mRNA was higher in RSA women. And the Interleukin 10 mRNA was lower, while the Interleukin 2 mRNA was higher in RSA women compared with the normal pregnant women. Conclusion The results suggest that in normal pregnancy main secretion is Interleukin 10. It may cause RSA, when Interleukin 10 mRNA decreases while Interleukin 2 mRNA increases in pregnant women.