Objective To study the sterilization efficiency of a new style slow-release disinfectant to total count of E.coli and bacteria.Methods The concentration of active chlorine was determined by iodination.The sterilization efficiency for E.coli was observed,including the influence of concentration of active chlorine(0.1,0.3 and 0.5 mg/L),pH value(6.5,7.0 and 8.5),water temperature(5,20 and 30 ℃) and chroma(0,15 and 20).Results When the concentration of active chlorine was over 0.3 mg/L,the sterilization efficiency was not affected by the factors which were studied.Conclusion The water disinfected by the slow-release disinfectant can meet water quality standard.