Optimization of primary culture technique for rat hippocampal neuron

Objective: To establish primary culture technique for rat hippocampal neuron.Methods:The neonatal Wistar rat(1~3d) were killed,the hippocampal tissue was separated and quickly broken into pieces in Hanks liquid,and then digested in 0.125% trypsinase and ended to be digested with fetal cattle serum.The cells were counted and then grew in culture plate which was coated with polylysine.Results:The neuron livability was high and the pure degree reach 90%,and the growth of neuron were good.Conclusion:Rat hippocampal neurons are obtained successfully by this way.