The application significance of TALEN technique for genome research

Gene knockout is a broadly applicable approach for efficiently editing any gene of cells and organisms. The transcription activator-like effector nuclease(TALEN) technique is a newly-developed method,and it can target essentially any sequence by comprising a nonspecific DNA-cleaving nuclease fused to a DNA-binding domain,which can be engineered easily. TALEN technique with the capability to quickly and efficiently alter genes have profound impacts on biological research and get potential therapeutic strategies for genetic diseases.