Study on Content Determination of Ursolic Acid and Oleanolic Acid in Cornus officinalisis

A RP-HPLC method for content determination of ursolic acid and oleanolic acid in Cornus officinalis was established.The Kromasil C18(250 mm×4.6 mm,5 μm) was used as analytical column with methanol-water-phosphate acid(90∶10∶0.2) as mobile phase.The detection wavelength was set at 210nm.The flow rate was 0.8 ml/min and the column temperature was 28 ℃.The calibration curve of ursolic acid was linear over the range of 0.67～6.03 μg and the correlation coefficient was 0.999 6.The calibration curve of oleanolic acid was linear over the range of 0.66～5.94 μg and the correlation coefficient was 0.999 6.The average recoveries of ursolic acid and oleanolic acid were 97.6 %(RSD= 1.6 %) and 99.8 %(RSD=2.1%),respectively.The method was simple,sensitive,accurate and reproducible.It could be used to control the quality of Cornus officinalis.