Black soldier fly antimicrobial peptides induced conditions optimization and research of crude extracts activity

Antimicrobial peptides were induced from black soldier fly(Hermetia illucens L.) larvae by different induction methods, doses and durations, We got crude antimicrobial peptides by grinding extraction method, we compared activity of those crude antimicrobial peptides, optimized induction conditions and provided reliable experimental conditions for separating and purifing the antimicrobial peptides.Our results demonstrated that the antimicrobial peptides extracted from different instar larvae had different antimicrobial activity, antimicrobial peptides extracted from 5th instar larvae had strongest antimicrobial activity.Diffenent inducing methods created different antimicrobial activity.When the larvae were induced by Escherichia coli liquid acupuncture for 60 s, their antimicrobial peptides had strongest antimicrobial activity, following was induced by 100W ultrasonic for 20 minutes, but their activity showed no significant difference.We also obtained the antimicrobial peptides Induced by Escherichia coli and Staphylococcus aureus liquid injection, Staphylococcus aureus liquid acupuncture and 40W ultrasonic, but those antimicrobial peptides had obviously weaker antimicrobial activity compared with the best method.