Highly Emissive Luminophores for Direct Chemiluminescence Detection

Direct chemiluminescence (CL) detection requires a proper luminophore to emit light through nonenzymatic activation. Herein, we synthesized a new class of 3-aminophthalhydrazide-type luminophores (LM-1–LM-13) with intensive CL emission. A typical analogue LM-5 was studied in detail, which exhibits greatly improved CL properties and enhanced storage stability compared to the widely used luminophore N-(4-aminobutyl)-N-ethylisoluminol (ABEI). The result of magnetic bead-based CL detection reveals that LM-5 holds great potential to become a next-generation luminophore in biosensing applications.