Optimization of preparation process of chitosan-nano-selenium

Compared with Inorganic Selenium and organic selenium, nano-selenium has the advantages of low toxicity, easy absorption, and high safety. However, due to their small size, the particles were easy to agglomerate and affected the biological efficiency. In this paper, the preparation process of nano-selenium optimized by Box-Behnken Design and used chitosan as biological template. The dosage of chitosan, temperature, Vc, disodium selenite, reaction time were investigated by particle size. The results show the best condition: chitosan of 0.006g, Vc dosage: disodium selenite dosage 5:1, temperature 35°C, with selenium nanoparticles of 116 nm.