重组酶聚合酶扩增
环介导等温扩增
数字聚合酶链反应
清脆的
核酸
微流控
多路复用
重组酶
数字微流体
PCR的应用
计算生物学
计算机科学
纳米技术
聚合酶链反应
材料科学
生物
DNA
生物信息学
重组
遗传学
光电子学
基因
电润湿
电介质
作者
Weihong Yin,Jianjian Zhuang,Jiale Li,Liping Xia,Kai Hu,Juxin Yin,Ying Mu
出处
期刊:Small
[Wiley]
日期:2023-08-23
卷期号:19 (49)
被引量:2
标识
DOI:10.1002/smll.202303398
摘要
Digital nucleic acid detection based on microfluidics technology can quantify the initial amount of nucleic acid in the sample with low equipment requirements and simple operations, which can be widely used in clinical and in vitro diagnosis. Recently, isothermal amplification technologies such as recombinase polymerase amplification (RPA), loop-mediated isothermal amplification (LAMP), and clustered regularly interspaced short palindromic repeats-CRISPR associated proteins (CRISPR-Cas) assisted technologies have become a hot spot of attention and state-of-the-art digital nucleic acid chips have provided a powerful tool for these technologies. Herein, isothermal amplification technologies including RPA, LAMP, and CRISPR-Cas assisted methods, based on digital nucleic acid microfluidics chips recently, have been reviewed. Moreover, the challenges of digital isothermal amplification and possible strategies to address them are discussed. Finally, future directions of digital isothermal amplification technology, such as microfluidic chip and device manufacturing, multiplex detection, and one-pot detection, are outlined.
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