Three undescribed coumarins from the roots of Angelica pubescens

AbstractThree undescribed coumarins, 6-(3-hydroxy-3-methyl-2-oxobutyl)-7-methoxycoumarin (1), (R)-6-(1,3-dihydroxy-3-methyl-2-oxobutyl)-7-methoxycoumarin (2), angelol N (6), together with four known coumarins were isolated from the roots of Angelica pubescens. Their structures were determined by extensive spectroscopic analyses. The absolute configurations of compounds 2 and 6 were assigned via electronic circular dichroism (ECD). Their inhibitory effects on lipopoly-saccharide (LPS)-induced nitric oxide (NO) production in RAW264.7 cells were evaluated. Compounds 1–4 exhibited moderate inhibitory activities.Keywords: Angelica pubescenscoumarinsECDRAW264.7 cells Disclosure statementNo potential conflict of interest was reported by the authors.Additional informationFundingThis work was supported by the Special Funds for transformation and upgrading of industrial informatization of industry and information technology department of Jiangsu in 2020 'Research on Key technologies of multi-component traditional Chinese medicines'.