Genome Mining and Molecular Networking Guided Isolation of Antimycin Analogs with Antifeedant Activities from the Deep‐Sea‐Derived Streptomyces sp. NA13

Abstract Polyphagous insects could affect agricultural production, which leads to serious economic losses. Due to the negative effects of synthesized insecticides, finding eco‐friendly and new biopesticides is emergent. To develop natural origin insecticides, an integrative approach combining antifeedant activity screening, genome mining, and molecular networking has been applied to discover antifeedant secondary metabolites from Streptomyces sp. NA13, which leads to the isolation of a novel antimycin Q ( 1 ) and six known antimycin analogs (antimycins A1a, A2a, A3a, A4a, A7a, and N ‐formylantimycic acid methyl ester, 2 – 7 ). Their structures were identified by high‐resolution mass spectrometry (HR‐MS) and nuclear magnetic resonance (NMR) spectroscopic. The absolute configuration of 1 was elucidated by the comparison of coupling constant, electronic circular dichroism (ECD) analysis, and NMR calculations. 1 – 6 exhibited different levels of antifeedant activities against Helicoverpa armigera , especially 1– 4 . At the same time, the antifeedant activity of antimycin was reported firstly.