拟杆菌
噬菌体
生物
拟杆菌
细菌
微生物学
脆弱类杆菌
拟杆菌
多糖
微生物群
生物化学
基因
遗传学
大肠杆菌
16S核糖体RNA
作者
Nathan T. Porter,Andrew J. Hryckowian,Bryan D. Merrill,Jaime J. Fuentes,Jackson O Gardner,Robert W. P. Glowacki,Shaleni Singh,Ryan Crawford,Evan S. Snitkin,Justin L. Sonnenburg,Eric C. Martens
出处
期刊:Nature microbiology
日期:2020-06-29
卷期号:5 (9): 1170-1181
被引量:78
标识
DOI:10.1038/s41564-020-0746-5
摘要
A variety of cell surface structures dictate interactions between bacteria and their environment, including their viruses (bacteriophages). Members of the human gut Bacteroidetes characteristically produce several phase-variable capsular polysaccharides (CPSs), but their contributions to bacteriophage interactions are unknown. To begin to understand how CPSs have an impact on Bacteroides–phage interactions, we isolated 71 Bacteroides thetaiotaomicron-infecting bacteriophages from two locations in the United States. Using B. thetaiotaomicron strains that express defined subsets of CPSs, we show that CPSs dictate host tropism for these phages and that expression of non-permissive CPS variants is selected under phage predation, enabling survival. In the absence of CPSs, B. thetaiotaomicron escapes bacteriophage predation by altering expression of eight distinct phase-variable lipoproteins. When constitutively expressed, one of these lipoproteins promotes resistance to multiple bacteriophages. Our results reveal important roles for Bacteroides CPSs and other cell surface structures that allow these bacteria to persist under bacteriophage predation, and hold important implications for using bacteriophages therapeutically to target gut symbionts. Isolation of phages associated with the gut commensal Bacteroides thetaiotaomicron reveals a link between cell surface structures, including phase-variable capsular polysaccharides, lipoproteins and S-layer proteins, and susceptibility to phage infection.
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