化学
适体
原位
循环肿瘤细胞
辣根过氧化物酶
检出限
电化学
全血
纳米技术
生物物理学
组合化学
分子生物学
电极
生物化学
色谱法
免疫学
酶
癌症
内科学
有机化学
材料科学
物理化学
生物
医学
转移
作者
Jianmei Yang,Xiaolong Li,Bingying Jiang,Ruo Yuan,Yun Xiang
标识
DOI:10.1021/acs.analchem.0c01195
摘要
Monitoring circulating tumor cells (CTCs) in human blood can offer useful information for convenient metastasis diagnosis, prognosis, and treatment of cancers. However, it remains a substantial challenge to detect CTCs because of their particular scarcity in complex peripheral blood. Herein, we describe an in situ-generated multivalent aptamer network-modified electrode interface for efficiently capturing and sensitively detecting CTCs in whole blood by electrochemistry. Such an interface was fabricated via rolling circle amplification extension of the electrode-immobilized primer/circular DNA complexes for the yield of long ssDNA strands with many repeated aptamer segments, which could achieve efficient capture of rare CTCs in a multivalent cooperative manner. The antibody and horseradish peroxidase-functionalized gold nanoparticles further specifically associated with the surface-bound CTCs and generated electrocatalytically amplified current outputs for highly sensitive detection of CTCs with an attractive detection limit of five cells. Also, the multivalent aptamer network interface could successfully distinguish the target cells from other control cells and achieve CTC detection in whole blood, demonstrating its promising potential for monitoring different rare CTCs in human blood.
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