银纳米粒子
寡核苷酸
化学
阳极溶出伏安法
超微电极
剥离(纤维)
水溶液中的金属离子
检出限
电化学
DNA
杂交探针
纳米颗粒
金属
分析化学(期刊)
电极
色谱法
循环伏安法
纳米技术
材料科学
生物化学
有机化学
物理化学
复合材料
作者
Hong Cai,Ying Xu,Ning Zhu,Pingang He,Yuzhi Fang
出处
期刊:Analyst
[The Royal Society of Chemistry]
日期:2002-05-01
卷期号:127 (6): 803-808
被引量:191
摘要
A novel, sensitive electrochemical DNA hybridization detection assay, using silver nanoparticles as the oligonucleotide labeling tag, is described. The assay relies on the hybridization of the target DNA with the silver nanoparticle-oligonucleotide DNA probe, followed by the release of the silver metal atoms anchored on the hybrids by oxidative metal dissolution and the indirect determination of the solubilized Ag(I) ions by anodic stripping voltammetry (ASV) at a carbon fiber ultramicroelectrode. The influence of the relevant experimental variables, including the surface coverage of the target oligonucleotide, the duration of the silver dissolution steps and the parameters of the electrochemical stripping measurement of the silver(I) ions, is examined and optimized. The combination of the remarkable sensitivity of the stripping metal analysis at the microelectrode with the large number of silver(I) ions released from each DNA hybrid allows detection at levels as low as 0.5 pmol L(-1) of the target oligonucleotides.
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