精液
运动性
精子库
医学
液氮
精子
男科
精子活力
低温保存
小瓶
生育率
生物
人口
色谱法
细胞生物学
化学
胚胎
环境卫生
有机化学
出处
期刊:JAMA
[American Medical Association]
日期:1973-02-12
卷期号:223 (7): 774-774
被引量:62
标识
DOI:10.1001/jama.1973.03220070030007
摘要
Human semen was frozen and stored in liquid nitrogen for periods of time up to eight years and two months. A total of 1,764 vials containing 1.1 ml of semen from 533 ejaculates provided by 207 donors were frozen. The freezing and thawing process induced a loss of approximately 50% of the original motility. Storage in liquid nitrogen for periods up to 36 months resulted in no further significant loss in mean motility. However, after this time, a progressive significant loss in motility was observed throughout the period of this study. Specimens with initial sperm counts between 40 and 200 million/ml and initial motilities between 60% and 89% appeared to withstand long-term storage best.
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