连接蛋白
生物
细胞培养
缝隙连接
肿瘤转化
北方斑点
抑癌基因
异源的
污渍
分子生物学
细胞内
基因表达
转染
细胞
细胞生物学
癌症研究
癌变
基因
遗传学
作者
Kimberley Cesen-Cummings,Martha J. Fernström,Alvin M. Malkinson,Randall J. Ruch
出处
期刊:Carcinogenesis
[Oxford University Press]
日期:1998-01-01
卷期号:19 (1): 61-67
被引量:87
标识
DOI:10.1093/carcin/19.1.61
摘要
The reduced gap junctional intercellular communication (GJIC) and gap junction protein (connexin) expression that have been noted in many neoplastic cell types may contribute to the neoplastic phenotype. We assessed GJIC (by fluorescent dye micro-injection) and connexin expression (by Northern blotting, Western blotting and immunohistochemistry) in five mouse and 17 human lung carcinoma cell lines; both measures were lower in neoplastic cells compared to non-transformed lung epithelial cells. Other connexins were not detected in these cells. Co-culture experiments indicated that carcinoma cell lines able to transfer dye among themselves (homologous GJIC) had little capacity for dye-coupling with non-transformed cells (heterologous GJIC). Southern blot analyses indicated that reductions in GJIC and connexin43 expression were not due to deletions or rearrangements of this gene, but were more likely accounted for by transcriptional down-regulation and/or post-transcriptional factors. No correlations between GJIC and known oncogene and tumor suppressor gene alterations in the human lung carcinoma cells were apparent, suggesting that other mechanisms down-regulate GJIC in these cells. Since the neoplastic cell lines exhibited low GJIC (either homologous or heterologous), this characteristic may be involved in expression of the neoplastic phenotype.
科研通智能强力驱动
Strongly Powered by AbleSci AI