磷酸化
糖皮质激素受体
内分泌学
内科学
雄激素受体
p38丝裂原活化蛋白激酶
雄激素
生物
激酶
MAPK/ERK通路
糖皮质激素
受体
医学
激素
生物化学
癌症
前列腺癌
作者
Justin X. Nicoll,Andrew C. Fry,Eric M. Mosier
出处
期刊:Steroids
[Elsevier]
日期:2019-01-01
卷期号:141: 23-29
被引量:14
标识
DOI:10.1016/j.steroids.2018.11.004
摘要
To determine if there is differential expression and phosphorylation of the androgen receptor (AR), glucocorticoid receptor (GR), and mitogen-activated protein kinases (MAPK) in skeletal muscle at rest between males and females. Ten college-aged males (mean ± SD; age = 22 ± 2.4 yrs, ht = 175 ± 7 cm, body mass = 84.1 ± 11.8 kg) and ten females (mean ± SD; age = 20 ± 0.9 yrs; ht = 169 ± 7 cm; body mass = 67.1 ± 8.7 kg) reported to the laboratory following an overnight fast. Resting muscle biopsies were collected from the vastus lateralis and analyzed for total and phosphorylated GR (ser134, ser211, and ser226), total and phosphorylated AR (ser81, ser213, ser515, ser650), and total and phosphorylated MAPK (ERK, JNK, p38) via western blotting. A phosphorylation index (PI) was calculated to determine phosphorylated receptor expression after accounting for differences in total receptor content. Males had more total AR compared to females (+42 ± 4%; p < 0.001). Females had higher phosphorylation of ARser81 (+87 ± 11%; p = 0.001) and ser515 (+55 ± 13%; p = 0.019). However, when the phosphorylated ratios were corrected for differences in total AR expression (i.e. our PI), the overall phosphorylation at these sites were similar between sexes (ser515, males = 100% vs females 92%; ser81, males = 100% vs females = 107%). pGRser134 was higher in males compared to females (+50 ± 15%; p = 0.016). Phospho-p38 was higher in females compared to males (+5050 ± 16%; p < 0.001). At rest, ARs and GRs are differentially phosphorylated at some, but not all sites when comparing males and females. Differential regulation of phosphorylated AR, GR, and p38 between males and females may have implications for the degree of muscle adaptations observed following resistance or endurance training.
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