生物
猪繁殖与呼吸综合征病毒
清脆的
病毒复制
病毒学
Cas9
基因组编辑
遗传学
基因组
病毒
基因
作者
Jingjing Jiang,Yuqiang Sun,Yunlong Wang,Ahmed Sabek,Aishao Shangguan,Kai Wang,Zhao Song,Guoliang Li,Ao Zhou,Shujun Zhang
出处
期刊:Virus Research
[Elsevier]
日期:2022-06-01
卷期号:314: 198738-198738
被引量:1
标识
DOI:10.1016/j.virusres.2022.198738
摘要
Porcine reproductive and respiratory syndrome (PRRS), a viral infection caused by PRRS virus (PRRSV) can result in severe reproductive failure, and respiratory disease in the pigs thus causing enormous economic losses to the global swine industry. Although the cellular receptors for PRRSV have been identified, but mechanisms underlying PPRSV replication remain obscure. Here, we have performed a genome-scale CRISPR/Cas9 knockout screen in the pig kidney cells with PRRSV. Several genes were found to be highly enriched post-PRRSV selection, just like KxDL Motif Containing 1(KXD1), Proteasome 26S Subunit, Non-ATPase 3 (PSMD3) and Galectin 2 (LGALS2) and soon on. Importantly, we have identified that loss of KXD1 resulted in the restricted autophagy and inhibited replication of PRRSV. Therefore, our study demonstrates that CRISPR/Cas9 system can be effectively used for the screening of pig factors responsible for PRRSV replication.
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