Modulation of the binding ability to biomacromolecule, cytotoxicity and cellular imaging property for ionic liquid mediated carbon dots

For the preparation of carbon dots (CDs), a variety of carbon sources and synthetic protocols are available which endow CDs with variable and unpredictable properties. In the present study, three CDs were developed with ionic liquid 1-butyl-3-methylimidazolium dicyanamide as the precursor through ethanol-thermal and hydrothermal strategies, termed as E-CDs and H-CDs, respectively. The features of these carbon dots, i.e., their physicochemical and optical properties, their interactions with bovine serum albumin (BSA) as well as their imaging capability were investigated with respect to the CDs prepared with microwave assisted approach (W-CDs). E-CDs and H-CDs were demonstrated to exhibit similar framework structures and optical properties, and they exhibited larger particle-sizes than that of W-CDs. In addition, the increase of ethanol-thermal and hydrothermal reaction time strengthened the quantum yields of the CDs and promoted their binding capability with BSA. E-CDs and H-CDs showed similar cytotoxicity on normal (LX-2) and cancer (SK-Hep-1) cells. We further found that these CDs may readily enter the cells within 5 min, while the fluorescence of hydrophilic E-CDs and H-CDs was very weak with respect to that of hydrophobic W-CDs in cell imaging. On the other hand, all the CDs exhibited little impact on the level of intracellular reactive oxygen species. The present study is conducive to guide the preparation of suitable carbon dots for different application scenarios.