线性判别分析
主成分分析
判别函数分析
色谱法
高效液相色谱法
多元统计
数学
模式识别(心理学)
聚类分析
人工智能
统计
化学
计算机科学
作者
Fuli Zhang,Wei Liu,Jianfei Mao,Qiuxiang Yin,Qing Lan,Qian Liu,Zhang YiRong,Longfei Chen,Xinling Yang,Xiang Luo,Min Chen,Ling-An Guo,Shaorong Lei
出处
期刊:China journal of Chinese materia medica
[China Journal of Chinese Materia Medica]
日期:2021-07-01
卷期号:46 (13): 3337-3348
被引量:2
标识
DOI:10.19540/j.cnki.cjcmm.20210316.101
摘要
A high performance liquid chromatography( HPLC) method was established for the fast,and precise determination of ten nucleosides in Fritillariae Cirrhosae Bulbus and its counterfeits. Then multivariate statistical analyses,such as clustering analysis,principal component analysis( PCA),and Fisher' s linear discriminant analysis( LDA),were conducted to establish a discriminant function model for an integrated analysis. The results indicated that data acquisition time of a single sample was shortened within 16 min by the HPLC method. In the range of 5-1 000 mg·kg~(-1),the mass concentrations of all nucleosides exhibited good linear relationships with the corresponding peak areas( R2> 0. 999). The spiked recoveries were in the range of 93. 83%-108. 9% with RSDs of0. 12%-1. 3%( n = 5). The limit of quantitation( LOQ) was 0. 98-4. 13 mg·kg~(-1). As revealed by the clustering analysis,Fritillariae Cirrhosae Bulbus and the counterfeits could be discriminated into two clusters based on the content of nucleosides. Fisher's LDA could achieve this discrimination,while PCA dimension reduction failed. The accuracy of the discriminant function model established on the screened characteristic indicators reached 97. 5%. The present study proposed a new identification method of Fritillariae Cirrhosae Bulbus with one-dimensional indicators,which is simple,accurate,and reliable. It can provide a scientific basis for further optimizing the identification techniques for Fritillariae Cirrhosae Bulbus and inspiration for quality control strategy development of Chinese medicinal materials.
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