清晨好,您是今天最早来到科研通的研友!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您科研之路漫漫前行!

Clinical efficacy of a novel, high-sensitivity HBcrAg assay in the management of chronic hepatitis B and HBV reactivation

乙型肝炎表面抗原 乙型肝炎 医学 病毒学 乙型肝炎病毒 病毒
作者
Tomoyuki Inoue,Shigeru Kusumoto,Etsuko Iio,S. Ogawa,Takanori Suzuki,Shusuke Yagi,Atsushi Kaneko,Kiyotaka Matsuura,Katsumi Aoyagi,Yasuhito Tanaka
出处
期刊:Journal of Hepatology [Elsevier]
卷期号:75 (2): 302-310 被引量:65
标识
DOI:10.1016/j.jhep.2021.02.017
摘要

A fully automated, novel high-sensitivity hepatitis B core-related antigen assay (iTACT-HBcrAg) has been developed. We demonstrate the clinical utility of iTACT-HBcrAg for monitoring chronic hepatitis B (CHB) and for the early detection of HBV reactivation.After fundamental assessments, the clinical performance of iTACT-HBcrAg was compared with other HBV markers. i) Serial sera, available from 161 HBeAg-negative patients with CHB and persistently undetectable HBV DNA, were measured by iTACT-HBcrAg and a conventional HBcrAg assay (G-HBcrAg). ii) Serial sera from 13 HBV-reactivated patients were measured by iTACT-HBcrAg and an ultra-high-sensitivity HBsAg immune complex transfer-chemiluminescent enzyme immunoassay (lower limit of detection; 0.0005 IU/ml, ICT-CLEIA) to compare HBV DNA detection. iii) To elucidate the various HBcrAg components detected by iTACT-HBcrAg, OptiPrep density gradient centrifugation analysis was performed on sera obtained before and after HBV reactivation.The analytical performance of iTACT-HBcrAg was satisfactory. The sensitivity of iTACT-HBcrAg (2.1 Log U/ml) was approximately 10-fold greater than that of G-HBcrAg (2.8 Log U/ml). i) HBcrAg was detectable in the sera of 97.5% (157/161) of patients with CHB by iTACT-HBcrAg, of whom 75.2% (121/161) had ≥2.8 Log U/ml HBcrAg and 22.4% (36/161) had 2.1-2.8 Log U/ml HBcrAg, which was undetectable by G-HBcrAg. ii) 9 and 2 of 13 HBV-reactivated patients were HBcrAg-positive by iTACT-HBcrAg before and at HBV DNA positivity, respectively; 7 and 4 were HBcrAg-positive by iTACT-HBcrAg before and at HBsAg-positivity by ICT-CLEIA, respectively. iii) The HBcrAg detected by iTACT-HBcrAg before HBV reactivation was contained in empty particles (22 KDa precore protein).iTACT-HBcrAg could be used to better monitor responses to anti-HBV treatments in HBeAg-negative patients and for the early detection of HBV reactivation.A fully automated, novel high-sensitivity hepatitis B core-related antigen assay (iTACT-HBcrAg) has been developed. iTACT-HBcrAg can be used to monitor HBeAg-negative patients with chronic hepatitis B, as well as for the early detection of HBV reactivation. iTACT-HBcrAg could be used as a general marker of disease progression and treatment response.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
652183758完成签到 ,获得积分10
5秒前
抹缇卡完成签到 ,获得积分10
23秒前
浩浩完成签到 ,获得积分10
25秒前
陆黑暗完成签到 ,获得积分10
27秒前
38秒前
科研佟完成签到 ,获得积分10
40秒前
cadcae完成签到,获得积分10
41秒前
迅速的念芹完成签到 ,获得积分10
53秒前
焚心结完成签到 ,获得积分10
1分钟前
六等于三二一完成签到 ,获得积分10
1分钟前
快乐的七宝完成签到 ,获得积分10
1分钟前
Ray完成签到 ,获得积分10
1分钟前
1分钟前
serenity完成签到 ,获得积分10
1分钟前
ChatGPT发布了新的文献求助10
1分钟前
瘦瘦煎饼完成签到 ,获得积分10
1分钟前
ChatGPT发布了新的文献求助10
1分钟前
三脸茫然完成签到 ,获得积分10
1分钟前
ChatGPT完成签到,获得积分10
1分钟前
研友完成签到 ,获得积分10
2分钟前
fffffffffffffff完成签到 ,获得积分10
2分钟前
8R60d8完成签到,获得积分0
2分钟前
lunyu完成签到 ,获得积分10
2分钟前
坚强志泽完成签到 ,获得积分10
2分钟前
俊逸的白梦完成签到 ,获得积分10
2分钟前
橘子海完成签到 ,获得积分10
2分钟前
Tianju完成签到,获得积分10
2分钟前
研友_Lmg1gZ完成签到,获得积分10
2分钟前
huazhangchina完成签到 ,获得积分10
3分钟前
高雯完成签到,获得积分10
3分钟前
芝诺的乌龟完成签到 ,获得积分0
3分钟前
张颖完成签到 ,获得积分10
3分钟前
欢呼的茗茗完成签到 ,获得积分10
3分钟前
丹妮完成签到 ,获得积分10
3分钟前
3分钟前
longlonglong完成签到,获得积分10
3分钟前
3分钟前
荔枝波波加油完成签到 ,获得积分10
3分钟前
Danny完成签到 ,获得积分10
3分钟前
Young完成签到 ,获得积分10
4分钟前
高分求助中
좌파는 어떻게 좌파가 됐나:한국 급진노동운동의 형성과 궤적 2500
Sustainability in Tides Chemistry 1500
TM 5-855-1(Fundamentals of protective design for conventional weapons) 1000
Cognitive linguistics critical concepts in linguistics 800
Threaded Harmony: A Sustainable Approach to Fashion 799
Livre et militantisme : La Cité éditeur 1958-1967 500
氟盐冷却高温堆非能动余热排出性能及安全分析研究 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3052644
求助须知:如何正确求助?哪些是违规求助? 2709863
关于积分的说明 7418252
捐赠科研通 2354395
什么是DOI,文献DOI怎么找? 1246007
科研通“疑难数据库(出版商)”最低求助积分说明 605951
版权声明 595921