Variability of dissolved organic matter in two coastal wetlands along the Changjiang River Estuary: Responses to tidal cycles, seasons, and degradation processes

Dissolved organic matter (DOM) in the coastal tidal marsh-estuary systems are complex mixtures with different source materials that vary with hydrological regimes, seasons, and environmental conditions and are modified by removal processes including photochemical and microbial degradations. Here, monthly surveys of DOM and its optical properties (i.e., absorbance and fluorescence of DOM) covering a complete semi-diurnal tidal cycle were conducted in two coastal marshes with distinct hydrological regimes (i.e., one freshwater and one brackish marsh) in the Changjiang River Estuary (CRE). Four fluorescent components were identified by excitation-emission matrix fluoresces combined with parallel factor analysis (EEMs-PARAFAC) as two terrestrial humic-like components and two autochthonous protein-like components. Results indicated that ebbing waters draining the marshes were consistently enriched with highly absorbing, more humic and highly aromatic DOM compared to the flood tidewaters. On a seasonal basis, DOM dynamics were largely modulated by the marsh productivity and the seasonal Changjiang runoff. Protein-like fluorophores, however, demonstrated a constant, less variable pattern on both the tidal and seasonal timescales. Onsite water incubation experiments with photochemical and microbial alterations revealed that photochemistry was primarily responsible for the removal of optically active components in the marsh DOM pools whereas the impact of microbes was minor. Principal component analysis (PCA) illustrated the processes regulating the DOM dynamics at the marsh-estuarine interface and allowed a clear distinction of samples between the two marshes, in addition to the samples under the influence of episodic weather events (i.e., Super Typhoon Lekima in summer 2009 and the basin-wide severe flood event occurred in summer 2020). This study underscores the importance of world large-rivers such as Changjiang on estuarine marsh DOM dynamics and also highlights the substantial heterogeneity of the marsh DOM across a river-dominated estuary.