体感系统
空间组织
神经科学
皮质(解剖学)
原位
生物
原位杂交
计算生物学
转录组
视皮层
化学
进化生物学
遗传学
基因表达
基因
有机化学
作者
Simone Codeluppi,Lars E. Borm,Amit Zeisel,Gioele La Manno,Josina Anna van Lunteren,Camilla I. Svensson,Sten Linnarsson
出处
期刊:Nature Methods
[Springer Nature]
日期:2018-10-30
卷期号:15 (11): 932-935
被引量:414
标识
DOI:10.1038/s41592-018-0175-z
摘要
Global efforts to create a molecular census of the brain using single-cell transcriptomics are producing a large catalog of molecularly defined cell types. However, spatial information is lacking and new methods are needed to map a large number of cell type-specific markers simultaneously on large tissue areas. Here, we describe a cyclic single-molecule fluorescence in situ hybridization methodology and define the cellular organization of the somatosensory cortex.
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