相位反转
膜
皱纹假丝酵母
材料科学
超滤(肾)
化学工程
接触角
纳米复合材料
热稳定性
纳米颗粒
脂肪酶
色谱法
纳米技术
化学
复合材料
有机化学
生物化学
工程类
酶
作者
Atefeh Zare,Abdol-Khalegh Bordbar,Amir Razmjou,Faranak Jafarian
标识
DOI:10.1016/j.jbiotec.2018.11.011
摘要
In this study, the modified nanocomposite membrane of polyethersulfone (PES) with NH2-MIL-101(Cr) as a metal-organic framework (MOF) is exploited for Candida rugosa lipase (CRL) immobilization. To that end, the various amounts of NH2-MIL-101(Cr) nanoparticles are blended into PES casting solution to fabricate ultrafiltration membrane via phase inversion technique. The incorporation efficiency of NH2-MIL-101(Cr) nanoparticles on the membrane morphology is investigated using various techniques, namely atomic force microscopy (AFM), X-ray diffraction (XRD), and contact angle goniometry. In terms of water pure flux and CRL immobilization efficiency, the best performance is observed for PES-NH2-MIL1% membrane. This bioactive membrane (CRL@GA@PES-NH2-MIL1%) displays an improvement in pH and thermal stability and separation performance that makes it a fruitful candidate for using in bioreactors. The examination of the wet- and dry-storage stability of CRL@GA@PES-NH2-MIL1% demonstrates the high stability for the wet bioactive membrane. The reusability inspection of CRL@GA@PES-NH2-MIL1% represents about 50% conservation of the residual activity after 12 sequential usage cycles.
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