炎症体
TLR4型
脂多糖
化学
分泌物
细胞生物学
NF-κB
炎症
细胞质
肿瘤坏死因子α
磷酸化
信号转导
蛋白质亚单位
生物化学
生物
免疫学
受体
基因
作者
Yalin Wang,Lifeng Lai,Liping Teng,Yuhong Li,Jin Cheng,Jinghua Chen,Chao Deng
标识
DOI:10.1016/j.ijbiomac.2019.01.022
摘要
Dictyophora indusiata polysaccharides (DIP) shows antioxidant, anti-tumor and immunostimulatory activities. However, the anti-inflammatory roles of DIP in NLRP3 inflammasome in LPS-stimulated macrophages are still not well defined. In this study, we investigated the mechanism of the anti-inflammatory activity of DIP in LPS-primed RAW264.7 macrophages. Our data showed that DIP inhibited NF-κB signal pathway via modulating TLR4 expression, phosphorylation of IκBα and nuclear translocation of NF-κB-p65 subunit. Meanwhile, DIP reduced inflammasome activation via decreasing NLRP3 expression in cytoplasmic pools, limiting self-assembly of NLRP3 inflammasome, as well as the subsequent activation of caspase-1 and the secretion of IL-1β and IL-18. For the first time, we show that the anti-inflammatory activity of DIP is mediated by inhibiting TLR4/NF-κB signal pathway and NLRP3 inflammasome activation during LPS-induced acute inflammation in RAW264.7 macrophages.
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