NADH- induced “kick-on” fluorescent probe validates crosstalk with redox regulator GSH

Abstract Herein we describe the NADH tracking ability of “kick-on” self-immolative fluorescent probe (PNADH) in live cells. The probe (PNADH) showed selective fluorescence emission at 552 nm in the presence of NADH upon excitation at 510 nm. The fluorescence intensity of probe PNADHwas ˜10-fold increased in the presence of 80 equivalents (400 μM) of NADH in PBS buffer at physiological condition. The probe PNADH is highly chemoselective toward NADH over the other competitive analytes. The probe PNADH has provided information of NADH in cancer cells such as HeLa, MDA-MB 231, and human normal fibroblast WI-38 cells. The biocompatible probe PNADH visualized dynamic changes of NADH attributed to the fluctuation of the specific substrates such as glucose, pyruvate and lactate in the glycolysis pathway. The first time we noticed that the extent of NADH-expression is decreased with upregulation of GSH in the live cells. The report on persistent crosstalk between NADH with redox regulator GSH motivate to search an antagonist for diseases associated with oxidative stress.