Study on chemical constituents from Corydalis Rhizoma in Pan'an

Objective To study the chemical constituents from Corydalis Rhizoma in Pan'an county as characteristically regional traditional Chinese medicinal materials.Methods The constituents were separated and purified by chromatography on silica gel and Sephadex LH-20 columns,as well as the chemical structures of alkaloids were determined by physicochemical properties and spectral data analyses.Results Twenty alkaloids were obtained and identified as tetrahydrocoptisine(1),tetrahydrocolumbamine(2),tetrahydropalmatine(3),corybulbine(4),isocorybulbine(5),8-trichloromethyl-7,8-dihydrocoptisine(6),corydaline(7),8-oxocoptisine(8),()-corypalmine(9),dehydrocorydaline(10),13-methylpalmatrubine(11),oxoglaucine(12),protopine(13),noroxyhydrastinine(14),tetrahydroberberine(15),didehydroglaucine(16),pontevedrine(17),coptisine(18),palmatine(19),and berberine(20).Conclusion Compounds 6,9,and 16 are isolated from Corydalis Rhizoma for the first time.All13C-NMR and 1H-NMR data of compounds 6 and 16 are assigned for the first time using 2D NMR.