Extraction and activity of polysaccharide from Hericium erinaceus

Aim To explore the best method of extracting polysaccharide from Hericium erinaceus and to study its immune-enhancing activity.Methods The extracted polysaccharide is gained by degrease,lixiviation,concentrating,sevag reagent′s getting rid of free protein and precipitation through ethanol.The activity of mice′s LAK cell was determined by 3H-TDR.Result The orthogonal experiment shows that the best process is temperature at 85℃,time being 4 h,water capacity of 150 ml.The volume ratio of polysaccharide solution to sevag reagent is 3.When the alcohol consistency comes up to 75% in the polysaccharide solution,the polysaccharide is precipitated out more.The Polysaccharide also has a role in activation of mice LAK and IL-2 cell,which could enhance the immune activity.Conclusion Polysaccharide from Hericium erinaceus can be extracted by this method.And the polysaccharide has a remarkable immune-enhanceing activity.