In-vitro differentiation of early pig spermatogenic cells to haploid germ cells

生物 精子发生 精子细胞 维甲酸 细胞生物学 精子 生殖细胞 倍性 下调和上调 细胞分化 男科 免疫学 遗传学 基因 内分泌学 医学
作者
Kun Yu,Yi Zhang,Bao‐Lu Zhang,Hanyu Wu,Wuqi Jiang,Su-Tian Wang,Deping Han,Yixun Liu,Zhengxing Lian,Shoulong Deng
出处
期刊:Molecular human reproduction [Oxford University Press]
卷期号:25 (9): 507-518 被引量:10
标识
DOI:10.1093/molehr/gaz043
摘要

Spermatogonial stem cells (SSCs) self-renew and contribute genetic information to the next generation. Pig is wildly used as a model animal for understanding reproduction mechanisms of human being. Inducing directional differentiation of porcine SSCs may be an important strategy in exploring the mechanisms of spermatogenesis and developing better treatment methods for male infertility. Here, we established an in-vitro culture model for porcine small seminiferous tubule segments, to induce SSCs to differentiate into single-tail haploid spermatozoa. The culture model subsequently enabled spermatozoa to express the sperm-specific protein acrosin and oocytes to develop to blastocyst stage after round spermatid injection. The addition of retinoic acid (RA) to the differentiation media promoted the efficiency of haploid differentiation. RT-PCR analysis indicated that RA stimulated the expression of Stra8 but reduced the expression of NANOS2 in spermatogonia. Genes involved in post-meiotic development, transition protein 1 (Tnp1) and protamine 1 (Prm1) were upregulated in the presence of RA. The addition of an RA receptor (RAR) inhibitor, BMS439, showed that RA enhanced the expression of cAMP responsive-element binding protein through RAR and promoted the formation of round spermatids. We established an efficient culture system for in-vitro differentiation of pig SSCs. Our study represents a model for human testis disease and toxicology screening. Molecular regulators of SSC differentiation revealed in this study might provide a therapeutic strategy for male infertility.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
五月拾旧完成签到,获得积分10
1秒前
风必发布了新的文献求助10
4秒前
沉默洋葱完成签到,获得积分10
5秒前
6秒前
8秒前
坦率的乐蕊完成签到 ,获得积分10
9秒前
辛勤白猫发布了新的文献求助10
10秒前
10秒前
小郭子发布了新的文献求助10
12秒前
ycw7777完成签到,获得积分10
13秒前
ZBY发布了新的文献求助20
13秒前
xiao给xiao的求助进行了留言
18秒前
风必完成签到,获得积分10
19秒前
橘子发布了新的文献求助10
19秒前
19秒前
24秒前
26秒前
27秒前
科目三应助橘子采纳,获得10
28秒前
普外科老白完成签到,获得积分10
29秒前
29秒前
30秒前
30秒前
30秒前
星子完成签到,获得积分10
32秒前
guoxiang发布了新的文献求助10
32秒前
33秒前
33秒前
慕青应助Fan采纳,获得10
34秒前
35秒前
踏实紫烟发布了新的文献求助10
35秒前
37秒前
37秒前
37秒前
索多倍发布了新的文献求助10
37秒前
39秒前
曾德帅完成签到 ,获得积分10
40秒前
41秒前
JAJ发布了新的文献求助10
42秒前
44秒前
高分求助中
Encyclopedia of Mathematical Physics 2nd edition 888
Introduction to Strong Mixing Conditions Volumes 1-3 500
Tip60 complex regulates eggshell formation and oviposition in the white-backed planthopper, providing effective targets for pest control 400
Optical and electric properties of monocrystalline synthetic diamond irradiated by neutrons 320
共融服務學習指南 300
Essentials of Pharmacoeconomics: Health Economics and Outcomes Research 3rd Edition. by Karen Rascati 300
Peking Blues // Liao San 300
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 物理 生物化学 纳米技术 计算机科学 化学工程 内科学 复合材料 物理化学 电极 遗传学 量子力学 基因 冶金 催化作用
热门帖子
关注 科研通微信公众号,转发送积分 3801417
求助须知:如何正确求助?哪些是违规求助? 3347057
关于积分的说明 10331788
捐赠科研通 3063333
什么是DOI,文献DOI怎么找? 1681602
邀请新用户注册赠送积分活动 807626
科研通“疑难数据库(出版商)”最低求助积分说明 763825