适体
结合亲和力
抗体
重组DNA
配体(生物化学)
化学
体外
指数富集配体系统进化
分子生物学
免疫系统
体内
计算生物学
生物
生物化学
受体
免疫学
遗传学
基因
核糖核酸
作者
Hongyu Wang,Curtis H. Lam,Xin Li,Derek West,Xianbin Yang
出处
期刊:Biochimie
[Elsevier]
日期:2018-02-01
卷期号:145: 125-130
被引量:31
标识
DOI:10.1016/j.biochi.2017.09.006
摘要
Specific, chemically modified aptamers (X-Aptamers) were identified against two immune checkpoint proteins, recombinant Programmed Death 1 (PD-1) and Programmed Death Ligand 1 (PD-L1). Selections were performed using a bead-based X-Aptamer (XA) library containing several different amino acid functional groups attached to dU at the 5-position. The binding affinities and specificities of the selected XA-PD1 and XA-PDL1 were validated by hPD-1 and hPD-L1 expression cells, as well as by binding to human pancreatic ductal adenocarcinoma tissue. The selected PD1 and PDL1 XAs can mimic antibody functions in in vitro assays.
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