28-Homobrassinolide alleviates oxidative stress in salt-treated maize (Zea mays L.) plants

超氧化物歧化酶 过氧化氢酶 丙二醛 过氧化物酶 愈创木酚 化学 氧化应激 扎梅斯 农学 植物 园艺 生物化学 生物
作者
Narinder Arora,Renu Bhardwaj,Priyanka Sharma,Hardesh Kumar Arora
出处
期刊:Brazilian Journal of Plant Physiology 卷期号:20 (2): 153-157 被引量:55
标识
DOI:10.1590/s1677-04202008000200007
摘要

The present investigation was undertaken to study the effects of 28-homobrassinolide on the activities of antioxidative enzymes such as superoxide dismutase (EC 1.15.1.1),guaiacol peroxidase (EC 1.11.1.7),catalase (EC 1.11.1.6),glutathione reductase (EC 1.6.4.2) and ascorbate peroxidase (EC 1.11.1.11),as well as protein and malondialdehyde concentrations in 30d-old plants of Zea mays L. grown under salt stress.The seeds were soaked in 28-homobrassinolide solutions (0, 10 -8 , 10 -6 and 10 -4 mM) for 12 h and then sown in the field in a randomized block layout.The blocks were salinised with NaCl at concentrations of 0, 25, 50 and 75 mM.The activities of antioxidative enzymes and protein concentration increased in 28homobrassinolide-treated plants.Despite the enhancement of enzyme activities under salt stress alone, lipid peroxidation increased and protein concentration decreased.However, pre-sowing treatments of 28-homobrassinolide further enhanced the activities of antioxidative enzymes in addition to lowering lipid peroxidation and increasing protein concentration, thus suggesting that 28-homobrassinolide can alleviate oxidative stress in salt-treated maize plants.Key words: antioxidative enzymes, brassinosteroids, lipid peroxidation, maize, salt stress 28-Homobrassinolídeo reduz o estresse oxidativo em plantas de milho (Zea mays L.) tratadas com sal: Objetivou-se estudar os efeitos do 28-homobrassinolídeo sobre as atividades de enzimas antioxidantes [dismutase do superóxido (EC 1.15.1.1),peroxidase do guaiacol (EC 1.11.1.7),catalase (EC 1.11.1.6),redutase da glutationa (EC 1.6.4.2) e peroxidase do ascorbato (EC 1.11.1.11)]e concentrações de proteínas e aldeído malônico em plantas de milho com 30 d de idade, cultivadas sob estresse salino.As sementes foram embebidas em soluções de 28-homobrassinolídeo (0, 10 -8 , 10 -6 and 10 -4 mM) por 12 h e, então, semeadas no campo, seguindo-se um desenho experimental de blocos ao acaso.Os blocos foram salinizados com NaCl a concentrações de 0, 25, 50 e 75 mM.As atividades das enzimas antioxidantes e a concentração protéica aumentaram nas plantas tratadas com 28-homobrassinolídeo.A despeito do aumento das atividades das enzimas sob estresse salino isoladamente, a peroxidação lipídica aumentou e a concentração protéica reduziu-se.Todavia, tratamentos de pré-emergência com 28-homobrassinolídeo promoveram aumentos adicionais
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