脱氧核酶
化学
脱甲基酶
生物化学
DNA
组蛋白
作者
Xiuyuan Wang,Shenghui Yu,Yuqiu He,Fuan Wang,Xiaoqing Liu
标识
DOI:10.1002/cjoc.202300633
摘要
Comprehensive Summary A label‐free deoxyribozyme (DNAzyme) biosensor for m 6 A demethylase activity detection is developed. When demethylase FTO (Fat mass and obesity‐associated protein), an important m 6 A demethylase of ALKBH demethylase family is present, the m 6 A group is specifically demethylated, and the cleavage activity of DNAzyme is restored. This allows the cleavage product that contains G‐quadruplex sequence to bind to thioflavin T (ThT) and generate fluorescence signals. The biosensor shows high specificity and sensitivity, and fast reaction speed. Our study demonstrates a new design of allosteric DNAzyme for sensing. This method represents the first label‐free nucleic acid biosensor for FTO assay, providing a feasible route towards inhibitors screening.
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