Dengue Virus Replicative‐Form dsRNA Is Recognized by Both RIG‐I and MDA5 to Activate Innate Immunity

ABSTRACT RIG‐I like receptors (RLRs) are a family of cytosolic RNA sensors that sense RNA virus infection to activate innate immune response. It is generally believed that different RNA viruses are recognized by either RIG‐I or MDA5, two important RLR members, depending on the nature of pathogen‐associated molecular patterns (PAMPs) that are generated by RNA virus replication. Dengue virus (DENV) is an important RNA virus causing serious human diseases. Despite extensive investigations, the molecular basis of the DENV PAMP recognized by the host RLR has been poorly defined. Here, we demonstrated that the DENV infection‐induced interferon response is dependent upon both RIG‐I and MDA5, with RIG‐I playing a predominant role. Next we purified the DENV PAMP RNA from the DENV‐infected cells, and demonstrated that the purified DENV PAMP is viral full‐length double‐stranded RNA bearing 5'ppp modifications, likely representing the viral replicative‐form RNA. Finally, we confirmed the nature of the DENV PAMP by reconstituting the viral replicative‐form RNA from in vitro synthesized DENV genomic RNA. In conclusion, our work not only defined the molecular basis of the RLR‐PAMP interaction during DENV infection, but also revealed the previously underappreciated recognition of a distinct moiety of the same PAMP by different RLRs in innate immunity against RNA viruses.