血管生成
伤口愈合
炎症
外体
巨噬细胞
光热治疗
巨噬细胞极化
M2巨噬细胞
微泡
新生血管
癌症研究
川地31
医学
免疫学
体外
化学
材料科学
纳米技术
小RNA
生物化学
基因
作者
Junkai Zeng,Zhenyu Sun,Feihui Zeng,Changjiang Gu,Xiongsheng Chen
标识
DOI:10.1016/j.mtbio.2023.100649
摘要
Due to local overactive inflammatory response and impaired angiogenesis, current treatments for diabetic wounds remain unsatisfactory. M2 macrophage-derived exosomes (MEs) have shown considerable potential in biomedical applications, especially since they have anti-inflammatory properties that modulate macrophage phenotypes. However, exosome-based strategies still have limitations, such as short half-lives and instability. Herein, we develop a double-layer microneedle-based wound dressing system ([email protected]) by encapsulating MEs in the needle tips and polydopamine (PDA) nanoparticles in backing layer to simultaneously suppress inflammation and improve angiogenesis at the wound site. In vitro, released MEs increased macrophage polarization towards the M2 phenotype. In addition, mild heat (40 °C) generated by the photosensitive PMN backing layer contributed to improved angiogenesis. More importantly, [email protected] also showed promising effects in diabetic rats. The uncontrolled inflammatory response at the wound site was inhibited by [email protected] during a 14-day period; in addition, MEs and the photothermal effects produced by PMN provided a combined proangiogenic effect by improving the expression of CD31 and vWF. Collectively, this study provides a simple and efficient cell-free strategy for suppressing inflammation and promoting vascular regeneration to treat diabetic wounds.
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