Quantitative proteomics investigating the intrinsic adaptation mechanism of Aeromonas hydrophila to streptomycin

Abstract Aeromonas hydrophila , a prevalent pathogen in the aquaculture industry, poses significant challenges due to its drug‐resistant strains. Moreover, residues of antibiotics like streptomycin, extensively employed in aquaculture settings, drive selective bacterial evolution, leading to the progressive development of resistance to this agent. However, the underlying mechanism of its intrinsic adaptation to antibiotics remains elusive. Here, we employed a quantitative proteomics approach to investigate the differences in protein expression between A. hydrophila under streptomycin (SM) stress and nonstress conditions. Notably, bioinformatics analysis unveiled the potential involvement of metal pathways, including metal cluster binding, iron‐sulfur cluster binding, and transition metal ion binding, in influencing A. hydrophila ’ s resistance to SM. Furthermore, we evaluated the sensitivity of eight gene deletion strains related to streptomycin and observed the potential roles of petA and AHA_4705 in SM resistance. Collectively, our findings enhance the understanding of A. hydrophila ’ s response behavior to streptomycin stress and shed light on its intrinsic adaptation mechanism.