检出限
纳米棒
线性范围
级联
连锁反应
化学
纳米技术
脱氧核酶
材料科学
组合化学
色谱法
光化学
作者
Jinyue Shi,Yujie Song,Yu Lin,Yeyu Wu,Hongzhen Luo,Jun Yan,Ke-Jing Huang,Xuecai Tan
标识
DOI:10.1016/j.snb.2023.134430
摘要
Cancer is a serious threat to human health, and early identification of cancer biomarkers is crucial for timely treatment. A dual-mode biosensing platform has been developed to identify colon carcinoma suppressor microRNA-199a (miRNA-199a). A biofuel cell is firstly constructed using hybridization chain reaction and catalytic hairpin assembly self-assembly. The anode is a flexible carbon cloth (CP) loaded with glucose oxidase-functionalized molybdenum disulfide nanorods (MoS2 nanorods), and the bio-cathode is made up of double-stranded deoxyribonucleotide chains generated by nucleic acid amplification technology. The linear range of the electrochemical method is 0.1 fM-100 pM, and the limit of detection is 24.1 aM (S/N = 3). The colorimetric method has a linear range of 0.1 fM-10000 pM with detection limit of 34.5 aM (S/N = 3). The combination of MoS2 nanorods, enzyme-free cascade signal amplification technology, and dual-mode detection strategy provides high sensitivity and accuracy, offering a promising method for accurate determination of cancer biomarkers.
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