化学
光热治疗
胶体金
核酸内切酶
纳米颗粒
生物传感器
适体
线性范围
离子强度
吸收(声学)
色谱法
生物物理学
检出限
DNA
分析化学(期刊)
纳米技术
水溶液
生物化学
分子生物学
光学
材料科学
物理
物理化学
生物
作者
Xianghui Li,Yuting Yang,Shuangmu Zhuo,Zhenyu Lin,Jianxin Chen
出处
期刊:Talanta
[Elsevier]
日期:2024-01-01
卷期号:266: 125003-125003
被引量:1
标识
DOI:10.1016/j.talanta.2023.125003
摘要
A colorimetric and photothermal dual readout biosensor for Flap endonuclease 1 (FEN1) quantification was developed on the basis of target-prevented gold nanoparticles (AuNPs) aggregation. The exposed 5'-flap of double-flap DNA substrate modified on SAMBs was firstly cleaved by FEN1. Large amount of cleaved 5'-flap remained in the supernatant after simple magnetic separation, which can adsorb on the surface of AuNPs and effectively prevent the dispersed AuNPs from aggregation under high ionic concentration, accompanied with the color changing of the system, which can be recognized by nake eyes easily. The absorption intensity at 528 nm shows a good linear relationship with the increasing FEN1 concentration from 5.0 × 10-3 to 3.1 × 10-2 U μL-1 with a LOD of 1.6 × 10-3 U μL-1 (S/N = 3). Given the aggregated AuNPs have higher photothermal effect than that of the dispersed AuNPs, the target-prevented AuNPs aggregation avoids a sharp increase of temperature for the system under the laser radiation. The temperature change is linearly correlated with the FEN1 concentration in the range of 3.1 × 10-3-6.1 × 10-2 U μL-1 with a LOD of 1.1 × 10-3 U μL-1. The whole detection process can be completed within 1 h. The proposed system had been applied to detect FEN1 concentration in serum samples with satisfied results, which can be applied in resource-limited area easily and quickly.
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