化学
电喷雾电离
色谱法
质谱法
共发射极
电喷雾
分析化学(期刊)
萃取电喷雾电离
电离
分辨率(逻辑)
聚糖
质谱中的样品制备
有机化学
离子
光电子学
生物化学
物理
人工智能
计算机科学
糖蛋白
作者
Melinda Wojtkiewicz,Sabarinath Peruvemba Subramanian,Rebekah L. Gundry
标识
DOI:10.1021/acs.analchem.3c03649
摘要
Microflow porous graphitized carbon liquid chromatography (PGC-LC) combined with negative mode ionization mass spectrometry (MS) provides high resolution separation and identification of reduced native N-glycan structural isomers. However, insufficient spray quality and low ionization efficiency of N-glycans present challenges for negative mode electrospray. Here, we evaluated the performance of a recently developed multinozzle electrospray source (MnESI) and accompanying M3 emitter for microflow PGC-LC-MS analysis of N-glycans in negative mode. In comparison to a standard electrospray ionization source, the MnESI with an M3 emitter improves signal intensity, identification, quantification, and resolution of structural isomers to accommodate low-input samples.
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