Generation of a Naïve Human scFv Phage Display Library and Panning Selection

Phage display antibody libraries have been successfully used as the essential tool to produce monoclonal antibodiesMonoclonal antibodies (mAB) against a plethora of targets ranging from diseases to native biologically important proteins as well as small molecules. It is well documented that diverse antibody genes are the major genetic source for the construction of a high-quality antibody libraryAntibody libraries and selection of high-affinity antibodies. Naïve antibody librariesAntibody libraries are derived using the IgM repertoire of healthy donors obtained from B-cells isolated from humanHuman peripheral blood mononuclear cell (PBMC). Single-chain fragment variable (scFv)Single-chain fragment variable (scFv) is a routinely used format due to its smaller size and preference for phage display. The process involves the use of a two-step cloning method for library construction. The protocol also covers the biopanning process for target positive clone selection.