类有机物
结直肠癌
Wnt信号通路
癌症研究
生物
癌症
MAPK/ERK通路
细胞生物学
信号转导
遗传学
作者
Ronghui Tan,Ze Zhang,Pei-Rong Ding,Yue Liu,Huidong Liu,Mengji Lu,Ye-Guang Chen
标识
DOI:10.1016/j.canlet.2024.216737
摘要
Although organoids derived from tumor tissues have been widely used in cancer research, it is a great challenge for cultured organoids to retain the characteristics of the original tumor tissues due to their heterogeneity. In this study, we explore organoid culture recipes to capture tumor features of colorectal cancers. We find that the activation of Wnt and EGF signaling and inhibition of BMP signaling are non-essential for the survival of most colorectal cancer organoids (CRCOs). We design a growth factor-reduced culture medium containing FGF10, A83-01 (TGF-β type I receptor inhibitor), SB202190 (p38 MAPK inhibitor), gastrin, and nicotinamide. Using this medium, we can maintain tumor features in long-term CRCO cultivation, as evidenced by histopathology, genetic stability, tumorigenicity, and response of clinical treatments. Our findings offer a reliable and economical strategy for CRCO culture, facilitating the utilization of organoids in colorectal cancer research and treatment.
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