警卫室
微管
拟南芥
细胞生物学
细胞骨架
干旱胁迫
结束语(心理学)
生物
植物
化学
生物物理学
细胞
生物化学
基因
政治学
突变体
法学
作者
Liru Dou,Xiangfeng Wang,Tonglin Mao
出处
期刊:Methods in molecular biology
日期:2023-01-01
卷期号:: 249-255
标识
DOI:10.1007/978-1-0716-2867-6_19
摘要
Plants must cope with diverse environmental stresses during growth and development, among which drought is one of the most concerning global threats. Recent studies have shown that the disassembly of the microtubule cytoskeleton plays an essential role during ABA-induced stomatal closure in response to drought stress. To facilitate studies on the mechanisms of ABA-induced microtubule rearrangement during stomatal closure, we describe procedures for observing guard cells treated with ABA, visualizing the microtubule cytoskeleton in guard cells, and their subsequent quantitative analysis. We include both representative images and the quantification results to illustrate these experiments.
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