Efficacy of Bacillus coagulans BC01 on loperamide hydrochloride-induced constipation model in Kunming mice

洛哌丁胺 胃动素 血管活性肠肽 医学 生理盐水 便秘 药理学 口服 生长抑素 甘丙肽 内科学 胃肠病学 腹泻 神经肽 受体
作者
Xu Zhang,Yafang Chen,Xin Ma,Yang Yu,Xueping Yu,Xiaoyong Chen,Huayi Suo
出处
期刊:Frontiers in Nutrition [Frontiers Media SA]
卷期号:9 被引量:8
标识
DOI:10.3389/fnut.2022.964257
摘要

In this study, the laxative effect of Bacillus coagulans BC01 (BC01) in mice was investigated using a functional constipation mouse model. Six-week-old male specific pathogen-free (SPF) Kunming mice were randomly divided into five groups: normal control group (saline), model group (loperamide hydrochloride), drug control group (bisacodyl), BC01 low-dose group (4.0 × 108 CFU/mL) and BC01 high-dose group (4.0 × 109 CFU/mL). Except for the normal group, the functional constipation model was established by administering 0.25 mL of a loperamide hydrochloride suspension (1 mg/mL) twice daily for four consecutive days by oral gavage. After modeling, the BC01 groups were administered 0.25 mL of BC01. The bisacodyl served as a control and was administered orally at a dose of 100 mg/kg, while the other groups were administered 0.25 mL of sterile saline. After 7 days of continuous administration, the experimental mice were again induced by loperamide hydrochloride. During this period, the mechanism of BC01 to improve constipation symptoms in mice was analyzed by measuring the changes in body weight, fecal water content, small intestine propulsion rate, histology of small intestinal tissue sections, fecal microbial diversity, serum indices, as well as mRNA and protein expression levels in the small intestinal tissue. BC01 was found to significantly promote the intestinal propulsion rate and increase the fecal water content in the mice. BC01 could also alleviates constipation by regulating gastrointestinal motility (substance P, motilin, endothelin-1, somatostatin, and vasoactive intestinal peptide), gene expression (c-Kit, SCF, COX-2, NF-κB, iNOS, and eNOS), intestinal inflammation (eNOS, iNOS, NF-κB), and the intestinal microbiota composition in the constipated mice. In addition, the high-dose BC01 treatment had the best preventive effect on constipation. BC01 is a probiotic strain to effectively relieve the adverse effects of constipation.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
jx完成签到 ,获得积分10
2秒前
等待冰露完成签到 ,获得积分10
2秒前
3秒前
维多利亚少年完成签到,获得积分10
3秒前
3秒前
liu1发布了新的文献求助20
4秒前
抠脚大汉完成签到,获得积分10
5秒前
豆芽完成签到,获得积分10
5秒前
6秒前
8秒前
毅诚菌完成签到,获得积分10
9秒前
文静的峻熙完成签到,获得积分10
10秒前
抠脚大汉发布了新的文献求助10
11秒前
11秒前
12秒前
12秒前
13秒前
13秒前
哎嘤斯坦完成签到,获得积分10
14秒前
14秒前
14秒前
shania发布了新的文献求助10
17秒前
养乐多完成签到,获得积分10
18秒前
豪哥发布了新的文献求助10
19秒前
19秒前
19秒前
fantexi113发布了新的文献求助10
20秒前
闪闪的正豪完成签到,获得积分10
20秒前
yqcsysu完成签到 ,获得积分10
20秒前
21秒前
小马甲应助LL采纳,获得10
22秒前
22秒前
FJ发布了新的文献求助10
24秒前
可爱的猪猪完成签到,获得积分10
24秒前
桐桐应助liuyuqin采纳,获得10
24秒前
24秒前
包容的琦发布了新的文献求助10
25秒前
27秒前
FashionBoy应助苏格拉没有底采纳,获得10
28秒前
我爱科研发布了新的文献求助10
28秒前
高分求助中
Teaching Social and Emotional Learning in Physical Education 900
Plesiosaur extinction cycles; events that mark the beginning, middle and end of the Cretaceous 500
Two-sample Mendelian randomization analysis reveals causal relationships between blood lipids and venous thromboembolism 500
Chinese-English Translation Lexicon Version 3.0 500
[Lambert-Eaton syndrome without calcium channel autoantibodies] 440
薩提亞模式團體方案對青年情侶輔導效果之研究 400
3X3 Basketball: Everything You Need to Know 310
热门求助领域 (近24小时)
化学 材料科学 医学 生物 有机化学 工程类 生物化学 纳米技术 物理 内科学 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 电极 光电子学 量子力学
热门帖子
关注 科研通微信公众号,转发送积分 2388339
求助须知:如何正确求助?哪些是违规求助? 2094631
关于积分的说明 5273688
捐赠科研通 1821452
什么是DOI,文献DOI怎么找? 908550
版权声明 559391
科研通“疑难数据库(出版商)”最低求助积分说明 485416