Uncovering blood–brain barrier permeability: a comparative study of machine learning models using molecular fingerprints, and SHAP explainability

This study illustrates the use of chemical fingerprints with machine learning for blood-brain barrier (BBB) permeability prediction. Employing the Blood Brain Barrier Database (B3DB) dataset for BBB permeability prediction, we extracted nine different fingerprints. Support Vector Machine (SVM) and Extreme Gradient Boosting (XGBoost) algorithms were used to develop models for permeability prediction. Random Forest recursive Feature Selection (RF-RFS) method was used for extracting informative attributes. An additional database was employed for the validation phase. The results indicate that all nine datasets achieved good performance in training, test and validation stages. We further took MACC Keys fingerprints, one of the best performing models for explainability analysis. For this purpose, we used SHapley Additive exPlanations (SHAP) analysis on this dataset for the identification of key structural features influencing BBB permeability prediction. These features include aliphatic carbons, methyl groups and oxygen-containing groups. This study highlights the effectiveness of different fingerprint descriptors in predicting BBB permeability. SHAP analysis provides value additions to the simulations. These simulations will be of significant help in drug discovery processes, particularly in developing Central Nervous System (CNS) therapeutics.