Streptavidin Fe2O3-gold nanoparticles functionalized theranostic liposome for antibiotic resistant bacteria and biotin sensing

Novel methods of sensing and treatment required to elicit potent humoral and cellular immune responses. Here, Streptavidin functionalized α-Fe2O3-Au nanoparticles (STV-Mag) loaded cationic carbomate cholesterol is used as a carrier to release antibacterial thymol drug for Staphylococcus aureus (S. aureus) infected Caenorhabditis elegans (C. elegans). Pertaining to theranostic applications, efficient antimicrobial activity, and non-stimulated drug release and biotin dependent S. aureus growth were studied in-vivo. While STV-Mag was tethered on mercaptobenzoic acid (MBA) molecular cushion for label free streptavidin-biotin electrochemical sensing, the STV-Mag-carbomate cholesterol (STV-Mag-cCHOL liposome) vesicle with loaded drug was tethered on MBA for non-stimulant drug release through specific cholesterol-S. aureus interaction and confirmed electrochemically. Selectivity was confirmed using other pathogens, E. coli, Proteus and Enterococcus bacterium through antimicrobial studies along with S. aureus. The biotin sensing showed linear range from 10-15 to 10-3 M, which was not obtained by conventional methods. Fourier-Transform Infra-red (FT-IR), X-ray diffraction (XRD), Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM) techniques were used to characterize the nanoparticulate system.