In this era of the global scurry for food security, aquaculture has emerged as the leader among the sectors providing a sustainable solution to feed the nine billion people by 2050. However, this challenge of sustainable intensification holds the problem of rising stressors for cultured fish. Being a cold-blooded aquatic vertebrate, feed remains the only input for economically addressing the menace of stress in cultured fishes. The use of aquafeed additives in fish nutrition is a relatively new frontier wherein the focus is mostly on ameliorating attributes other than growth. Probiotics, prebiotics, synbiotics, vitamins, and amino acids are the few most important feed additives being used nowadays for stress amelioration in fish. This chapter focuses on the individual mechanisms and case-to-case studies by authors across the globe to give a holistic view of the use of aquafeed additives for stress amelioration in teleosts.